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Before All Grown Up!, a few Rugrats stories touched on attempts to either differentiate the twins personality-wise ("Twins Pique", 1993), or physically separate them ("Together at Last", 1992).
However, separating the twins only got serious in the new spin-off. Its premiere, "Coup DeVille" (first US tx: April 12, 2003), was focused on the twins. Lil strongly retaliated at being paired with Phil on a science project, as well as being treated "like a single package" with Phil, and even refused a trip to Twins Canyon with her family on that principle. This episode also repeated the "Lil is older" fact from the original series, and also revealed Lil to have an even longer name than was previously let on: Lillian Marie Jill DeVille. Lil moved to a separate bedroom in this episode.
Since then, the twins have evolved distinctly different personalities. Phil still enjoys repulsive objects (although he is beyond eating worms and insects, he once won a science fair by growing mold on cheese and socks), and has been referred to as "socially inept", while Lil is beginning to find interest in boys and become more social, and she has also picked up an interest in sports, particularly soccer.
Referencing their baby days when their favorite thing was to eat worms, Phil & Lil's favorite food in All Grown Up is Spaghetti.
In the episode Susie Goes Bad Lite, it is revealed that Phil is a good cook.